{"product_id":"cst-71128t","title":"CST,  71128T, TET1 (E2J2D) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying TET1 mouse. Validated for Western Blotting,CUT\u0026amp;RUN. Available in 2 sizes. Highly specific and rigorously validated in-house, TET1 (E2J2D) Rabbit Monoclonal Antibody (CST #71128) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nThe CUT\u0026amp;RUN dilution was determined using CUT\u0026amp;RUN Assay Kit # 86652 .\nWestern Blotting: 1:1000\nCUT\u0026amp;RUN: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, CUT\u0026amp;RUN\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nTET1 (E2J2D) Rabbit Monoclonal Antibody recognizes endogenous levels of total TET1 protein.\nSpecies Reactivity: Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse TET1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nMethylation of DNA at cytosine residues is a heritable, epigenetic modification that is critical for proper regulation of gene expression, genomic imprinting, and mammalian development (1,2). 5-methylcytosine is a repressive epigenetic mark established by two enzymes, DNMT3a and DNMT3b, and is maintained by DNMT1 (3,4). 5-methylcytosine was originally thought to be passively depleted during DNA replication. However, subsequent studies have shown that Ten-Eleven Translocation (TET) proteins TET1, TET2, and TET3 can catalyze the oxidation of methylated cytosine to 5-hydroxymethylcytosine (5-hmC) (5). Additionally, TET proteins can further oxidize 5-hmC to form 5-formylcytosine (5-fC) and 5-carboxylcytosine (5-caC), both of which are excised by thymine-DNA glycosylase (TDG), effectively linking cytosine oxidation to the base excision repair pathway and supporting active cytosine demethylation (6,7). TET1 is highly expressed in embryonic stem cells and is essential for maintaining stem cell pluripotency through demethylation of the promoter (8). Aberrant TET1 expression has also been implicated in a variety of cancers, including hepatocellular carcinoma, T-cell acute lymphoblastic leukemia (T-ALL), and triple-negative breast cancer (TNBC), among others (9-11).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n2510010B09Rik; AA517754; BB001228; CXXC finger 6; CXXC-type zinc finger protein 6; Cxxc6; D10Ertd17; D10Ertd17e; Kiaa1676; LCX; Methylcytosine dioxygenase TET1; mKIAA1676; Ten-eleven translocation 1 gene protein homolog; tet methylcytosine dioxygenase 1; tet oncogene 1; Tet1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M\nSENSITIVITY: Endogenous\nMW (kDa): 300\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800115761321,"sku":"71128T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-71128t","provider":"Iright","version":"1.0","type":"link"}