{"product_id":"cst-71184t","title":"CST,  71184T, Tpl2 (E1R5H) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Tpl2\/Cot. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Tpl2 (E1R5H) Rabbit Monoclonal Antibody (CST #71184) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nTpl2 (E1R5H) Rabbit Monoclonal Antibody recognizes endogenous levels of total Tpl2 protein. This antibody detects both isoform 1 (58 kDa) and isoform 2 (52 kDa) of Tpl2. A band of unknown origin was observed at 110 kDa.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human Tpl2 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nTpl2 (tumor progression locus 2), also known as COT (cancer osaka thyroid), is a serine\/threonine kinase expressed primarily in hematopoietic tissues, lung and liver (1). Over-expression of Tpl2 potentiates MAP kinase pathways through MEK1 and SEK1, as well as through MKK6 and MEK5 (2,3). Tpl2 is also engaged in NF-κB activation through NF-κB inducing kinase (NIK), or by inducing phosphorylation and degradation of the NF-κB precusor, p105 NF-κB1 (4,5). Ser400 of Tpl2 is phosphorylated in an Akt-dependent manner. This phosphorylation is required for Tpl2-induced NF-κB-dependent transcription (6). Tpl2 also activates caspase-3 by promoting the assembly of a protein complex of Apaf1 (apoptotic protease-activating factor 1), caspase-9, Tpl2, adaptor protein Tvl1 and procaspase-3 (7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\naugmented in rheumatoid arthritis 2; AURA2; c-COT; Cancer Osaka thyroid oncogene; COT; cot (cancer Osaka thyroid) oncogene; EST; ESTF; Ewing sarcoma transformant; FLJ10486; M3K8; MAP3K8; MEKK8; Mitogen-activated protein kinase kinase kinase 8; Proto-oncogene c-Cot; proto-oncogene serine\/threoine protein kinase; Serine\/threonine-protein kinase cot; TPL-2; TPL2; Tumor progression locus 2; tumor progression locus-2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nMW (kDa): 52, 58\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799872000169,"sku":"71184T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-71184t","provider":"Iright","version":"1.0","type":"link"}