{"product_id":"cst-71464s","title":"CST,  71464S, BCL6 (D4I2V) Rabbit Monoclonal Antibody (Biotinylated)","description":"Monoclonal Antibody for studying BCL6. Validated for Western Blotting. Highly specific and rigorously validated in-house, BCL6 (D4I2V) Rabbit Monoclonal Antibody (Biotinylated) (CST #71464) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 140 mM NaCl, 3 mM KCI, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg\/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nBCL6 (D4I2V) Rabbit Monoclonal Antibody (Biotinylated) recognizes endogenous levels of total BCL6 protein. This antibody is predicted to detect both human BCL6 isoform 1 and isoform 2.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a recombinant protein fragment spanning residues Asp210 through Thr409 of human BCL6 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nChromosomal translocations result in misregulation of the proto-oncogene in patients with B cell-derived non-Hodgkin's lymphoma (1). The gene is selectively expressed in mature B cells and encodes a nuclear phosphoprotein that belongs to the BTB\/POZ zinc finger family of transcription factors (2,3). BCL6 protein can bind to target DNA sequences of Stat6 and, analogous to Stat6, modulate the expression of interleukin-4-induced genes (4). Furthermore, BCL6 restrains p53-dependent senescence, making BCL6-active tumors functionally p53-negative (5). The mitogen-activated protein kinases, Erk1 and Erk2, but not JNK, phosphorylate BCL6 at multiple sites. Phosphorylation of BCL6 at Ser333 and Ser343 results in degradation of BCL6 by the ubiquitin\/proteasome pathway in B cells (6,7). In addition, BCL6 is acetylated and its transcriptional repressor function is inhibited by the transcriptional co-activator p300 (8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nB cell CLL\/lymphoma 6; B-cell CLL\/lymphoma 6; B-cell lymphoma 5 protein; B-cell lymphoma 6 protein; B-cell lymphoma 6 protein transcript; BCL-5; BCL-6; BCL5; BCL6; BCL6 transcription repressor; BCL6A; cys-his2 zinc finger transcription factor; LAZ3; lymphoma-associated zinc finger gene on chromosome 3; Protein LAZ-3; ZBTB27; Zinc finger and BTB domain-containing protein 27; Zinc finger protein 51; zinc finger transcription factor BCL6S; ZNF51\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 87-98\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800517955753,"sku":"71464S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-71464s","provider":"Iright","version":"1.0","type":"link"}