{"product_id":"cst-72099t","title":"CST,  72099T, LIME1 (E3M3B) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying LIME1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, LIME1 (E3M3B) Rabbit Monoclonal Antibody (CST #72099) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nLIME1 (E3M3B) Rabbit Monoclonal Antibody recognizes endogenous levels of total LIME1 protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro211 of human LIME1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nLck-interacting transmembrane adaptor 1 (LIME1) is a type III transmembrane adaptor protein that participates in lymphocyte signaling and is predominantly expressed in T lineage cells and plasma cells (1,2). LIME1 also harbors a palmitoylation motif for targeting to lipid rafts and potential tyrosine phosphorylation sites that are engaged upon CD4 and CD8 cross-linking (1). Research studies have demonstrated that in response to T cell receptor (TCR) engagement, LIME1 expression is upregulated, undergoes tyrosine phosphorylation, and associates with numerous signaling proteins such as Lck, GADS, and Vav. Collectively, these observations point toward a role for LIME1 in the regulation of T cell activation and adhesion (3,4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\ndJ583P15.4; FLJ20406; Lck interacting transmembrane adaptor 1; Lck-interacting membrane protein; Lck-interacting molecule; Lck-interacting transmembrane adapter 1; LIME; LIME1; LP8067\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 30\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799876784297,"sku":"72099T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-72099t","provider":"Iright","version":"1.0","type":"link"}