{"product_id":"cst-72105t","title":"CST,  72105T, FXR\/NR1H4 (E4B8P) Mouse Monoclonal Antibody","description":"Monoclonal Antibody for studying NR1H4. Validated for Western Blotting,Immunoprecipitation,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, FXR\/NR1H4 (E4B8P) Mouse Monoclonal Antibody (CST #72105) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits.\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:200\nChromatin IP: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Chromatin IP\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nFXR\/NR1H4 (E4B8P) Mouse Monoclonal Antibody recognizes endogenous levels of total FXR\/NR1H4 protein.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human FXR\/NR1H4 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe farnesoid X receptor (FXR\/NR1H4) is a member of the nuclear hormone receptor superfamily and is a master regulator of bile acid synthesis. FXR\/NR1H4 heterodimerizes with RXR-alpha upon activation by bile acids, which begins a regulatory cascade involving SHP and LRH-1 to control lipid homeostasis (1-5). FXR\/NR1H4 has also been shown to be a critical regulator of glucose homeostasis (6,7). In addition to directly regulating genes, FXR\/NR1H4 also plays a post transcriptional role in bile acid metabolism by transcribing the RNA-binding protein ZFP36L1, which in turn downregulates the key enzyme Cyp7a1 (8). Mutations in human FXR\/NR1H4 have been shown to cause cholestasis and liver disease in neonatal patients (9). FXR\/NR1H4 can also control Lgr5+ intestinal stem cell proliferation and its upregulation has been shown to inhibit colorectal cancer progression (10-12). Agonists against FXR\/NR1H4 are being evaluated for various liver diseases and diabetes (13-16).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nBAR; Bile acid receptor; farnesoid X nuclear receptor; farnesoid X receptor; Farnesoid X-activated receptor; Farnesol receptor HRR-1; FXR; HRR-1; HRR1; MGC163445; NR1H4; Nuclear receptor subfamily 1 group H member 4; nuclear receptor subfamily 1, group H, member 4; PFIC5; Retinoid X receptor-interacting protein 14; RIP14; RXR-interacting protein 14\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nMW (kDa): 55\nSource\/Isotype: Mouse IgG2a","brand":"CST","offers":[{"title":"Default Title","offer_id":46799877079209,"sku":"72105T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-72105t","provider":"Iright","version":"1.0","type":"link"}