{"product_id":"cst-72133s","title":"CST,  72133S, MLXIP\/MondoA (F8A8L) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying MLXIP. Validated for WB,WB,IP,ChIP. Available in 2 sizes. Highly specific and rigorously validated in-house, MLXIP\/MondoA (F8A8L) Rabbit Monoclonal Antibody (CST #72133) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP results, use 5 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits.\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\nImmunoprecipitation: 1:50\nChromatin IP: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Chromatin IP\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nMLXIP\/MondoA (F8A8L) Rabbit Monoclonal Antibody recognizes endogenous levels of total MLXIP\/MondoA protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MLXIP\/MondoA protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nMLX-interacting protein (MLXIP), also known as the transcriptional activator MondoA, forms a heterodimer with MLX to bind to DNA, activating transcription of genes involved in glycolysis (1,2). Studies show that MLXIP\/MondoA functions as a metabolic stress sensor in common B-cell acute lymphoblastic leukemia (B-ALL) cells by downregulating pyruvate dehydrogenase activity, inhibiting oxidative phosphorylation. Furthermore, MLXIP\/MondoA overexpression correlates with poor survival rates in pediatric common acute lymphoblastic leukemia, suggesting that MLXIP\/MondoA contributes to B-ALL malignancy by mediating adaptive responses to metabolic stress (3). In addition, studies show that MLXIP\/MondoA is a regulator of cellular senescence. It delays cellular senescence by maintaining mitochondrial homeostasis and activating autophagy (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nBHLHE36; Class E basic helix-loop-helix protein 36; KIAA0867; MIR; MLX interacting protein; Mlx interactor; MLX-interacting protein; MLXIP; MONDOA; Transcriptional activator MondoA\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 130\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799876980905,"sku":"72133S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-72133s","provider":"Iright","version":"1.0","type":"link"}