{"product_id":"cst-72364l","title":"CST,  72364L, SS18-SSX (E9X9V) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying SS18\/SSX1 fusion. Validated for WB,WB,IP,IHC,IHC,ChIP,ChIP. Available in 3 sizes. Highly specific and rigorously validated in-house, SS18-SSX (E9X9V) Rabbit Monoclonal Antibody (CST #72364) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 × 10^6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits.\nWestern Blotting: 1:1000\nSimple Western™: 1:50 - 1:250\nImmunoprecipitation: 1:100\nIHC Leica Bond: 1:200 - 1:800\nImmunohistochemistry (Paraffin): 1:400 - 1:1600\nChromatin IP: 1:50\nChromatin IP-seq: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier-free (BSA and azide free) version of this product see product # 92761 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, IHC Leica Bond, Immunohistochemistry (Paraffin), Chromatin IP, Chromatin IP-seq\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nSS18-SSX (E9X9V) Rabbit Monoclonal Antibody recognizes endogenous levels of total SS18-SSX fusion protein. This antibody does not cross-react with wild-type SS18 or SSX proteins.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the fusion site of human SS18-SSX protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nSS18 is a protein that has been shown to be a part of the SWI\/SNF complex (1, 2). The SS18-SSX fusion proteins are a result of in-frame fusions that fuse the gene on chromosome 18 with X chromosome genes , , and to a lesser extent (3). Human synovial sarcoma (SS) accounts for 8-10% of all soft tissue malignancies and 95% of these malignancies express the recurrent translocation of the gene on chromosome 18 (3). The N-terminal SNH domain (SYT N-terminal homology domain) of the SS18 protein interacts with SWI\/SNF chromatin remodeling complexes via the N terminal region of BRM and BRG1 subunits (4). Studies of the SS18-SSX fusion in SS suggest that endogenous SS18 competes with the mutant SS18-SSX fusion for occupancy in the SWI\/SNF complexes resulting in the displacement of SNF5 (BAF47) subunit. Displacement of the SNF5 subunit results in altered function of the SWI\/SNF complex that leads to deregulated expression of genes such as in SS (3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nFusion protein SYT-SSX1; SS18\/SSX1 fusion; SSX1\/SS18 fusion; SSX1\/SSXT fusion; SSXT\/SSX1 fusion; SYT-SSX1; SYT\/SSX1 fusion\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 65, 75\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799877931177,"sku":"72364L","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-72364l","provider":"Iright","version":"1.0","type":"link"}