{"product_id":"cst-72737s","title":"CST,  72737S, Phospho-RRM2 (Thr33) (F1B6L) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying RRM2 (Thr33) phosphate mouse. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Phospho-RRM2 (Thr33) (F1B6L) Rabbit Monoclonal Antibody (CST #72737) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-RRM2 (Thr33) (F1B6L) Rabbit Monoclonal Antibody recognizes endogenous levels of RRM2 protein only when phosphorylated at Thr33. By western blot, this antibody detects a band of unknown origin at approximately 75 kDa.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr33 of mouse RRM2 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nRibonucleotide reductase catalyzes the rate-limiting step in the synthesis of deoxynucleotide triphosphates (dNTPs). Ribonucleoside-diphosphate reductase subunit M2 (RRM2) is frequently overexpressed and associated with poor prognosis in multiple human cancers (1). RRM2\/AKT\/NF-κB signaling pathway is implicated in tumor invasiveness in gastric cancer (2). RRM2 is highly expressed in melanoma, and correlated with poor prognosis in BRAF-mutant melanoma. Knockdown of RRM2 stabilized the transient response of cells and patient-derived xenograft (PDX) model system to BRAF inhibition (3). Cyclin-dependent kinase (CDK) mediated phosphorylation of RRM2 at Thr33 targets the protein for degradation, allowing cells to maintain balanced dNTP pools (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAA407299; MGC113712; R2; ribonucleoside-diphosphate reductase M2 chain; Ribonucleoside-diphosphate reductase subunit M2; ribonucleotide reductase M2; Ribonucleotide reductase small chain; Ribonucleotide reductase small subunit; RIR2; Rrm2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 45\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799879405737,"sku":"72737S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-72737s","provider":"Iright","version":"1.0","type":"link"}