{"product_id":"cst-7299s","title":"CST,  7299S, PathScan® Phospho-c-Kit (Tyr719) Sandwich ELISA Antibody Pair","description":"ELISA Antibody Pair for studying Kit (Tyr721) phosphate in the research area.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nAntibody pairs have been optimized using recommended buffers, reagents, plates and the included protocol. Solutions should be made fresh daily.\n\u003cb\u003eStorage\u003c\/b\u003e\nCapture and detection antibodies are stored at 4°C. HRP-linked secondary reagent is stored at -20°C.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: ELISA+\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nFor Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan\nSpecies Reactivity: Human\n\u003cb\u003eBackground\u003c\/b\u003e\nc-Kit is a member of the subfamily of receptor tyrosine kinases that includes PDGF, CSF-1, and FLT3\/flk-2 receptors (1,2). It plays a critical role in activation and growth in a number of cell types, including hematopoietic stem cells, mast cells, melanocytes, and germ cells (3). Upon binding with its stem cell factor (SCF) ligand, c-Kit undergoes dimerization\/oligomerization and autophosphorylation. Activation of c-Kit results in the recruitment and tyrosine phosphorylation of downstream SH2-containing signaling components, including PLCγ, the p85 subunit of PI3 kinase, SHP2, and CrkL (4). Molecular lesions that impair the kinase activity of c-Kit are associated with a variety of developmental disorders (5), and mutations that constitutively activate c-Kit can lead to pathogenesis of mastocytosis and gastrointestinal stromal tumors (6). Tyr719 is located in the kinase insert region of the catalytic domain. c-Kit phosphorylated at Tyr719 binds to the p85 subunit of PI3 kinase and (7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nc-kit; cell based assay; cell-based assay; kit; mast\/stem cell growth factor receptor; scfr; screening\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H","brand":"CST","offers":[{"title":"Default Title","offer_id":46800650993833,"sku":"7299S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-7299s","provider":"Iright","version":"1.0","type":"link"}