{"product_id":"cst-73315sf","title":"CST,  73315SF, Cox2 (D5H5) Rabbit Monoclonal Antibody (BSA and Azide Free)","description":"Monoclonal Antibody for studying cyclooxygenase-2\/Cox-2. Validated for WB,IHC,IF,F,ELISA+. Highly specific and rigorously validated in-house, Cox2 (D5H5) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #73315) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nThis product is the carrier free version of product #12282. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT\u0026amp;RUN or CUT\u0026amp;Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions\/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.\n\u003cb\u003eFormulation\u003c\/b\u003e\nSupplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 12282\n\u003cb\u003eStorage\u003c\/b\u003e\nStore at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze\/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCox2 (D5H5) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total Cox2 protein. Species cross-reactivity for IF-IC is mouse and rat only. Non-specific labeling of centrioles may be observed by immunofluorescence in human cells.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His108 of human Cox2 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCyclooxygenase1 (Cox1) and cyclooxygenase2 (Cox2), family members with 60% homology in humans, catalyze prostaglandin production from arachidonic acid (1,2). While Cox1 expression is constitutive in most tissues, Cox2 expression is induced by lipopolysaccharide (LPS) and peptidoglycan (PGN) (3). PGN activates Ras, leading to phosphorylation of Raf at Ser338 and Erk1\/2 at Tyr204. The activation of MAP kinase signaling results in subsequent activation of IKKα\/β, phosphorylation of IκBα at Ser32\/36, and NF-κB activation. Finally, activation of the transcription factor NF-κB is responsible for the induction of Cox2 expression (4). Investigators have shown that LPS and PGN induce the clinical manifestations of arthritis and bacterial infections, such as inflammation, fever, and septic shock (5). Research studies have indicated that Cox1 and Cox2 may also play a role in the neuropathology of Alzheimer's disease by potentiating γ-secretase activity and β-amyloid generation (6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCOX-2; COX2; cyclooxygenase 2; cyclooxygenase 2b; Cyclooxygenase-2; GRIPGHS; hCox-2; PGG\/HS; PGH synthase 2; PGH2; PGHS-2; PHS II; PHS-2; Prostaglandin G\/H synthase 2; prostaglandin G\/H synthase and cyclooxygenase; Prostaglandin H2 synthase 2; Prostaglandin-endoperoxide synthase 2; prostaglandin-endoperoxide synthase 2 (prostaglandin G\/H synthase and cyclooxygenase); PTGS2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 74\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799883075753,"sku":"73315SF","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-73315sf","provider":"Iright","version":"1.0","type":"link"}