{"product_id":"cst-73828s","title":"CST,  73828S, INO80 (F2I3E) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying INOC1. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, INO80 (F2I3E) Rabbit Monoclonal Antibody (CST #73828) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nINO80 (F2I3E) Rabbit Monoclonal Antibody recognizes endogenous levels of total INO80 protein. This antibody detects bands of unknown origin at 145, 55, and 50 kDa.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly1547 of human INO80 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe INO80 complex is an ATP-dependent chromatin remodeling complex critical for many functions, including transcriptional regulation and DNA repair. INO80 is a large multi-subunit complex consisting of the key ATPase INO80 along with RUVBL1, RUVBL2, ARP4\/5\/8, and others (1-3). INO80 prefers binding to hexasomes and can facilitate the exchange of H2A.Z\/H2B dimers with H2A\/H2B (4-6). The INO80 complex is recruited to double-strand breaks (DSBs) through interaction between gamma-H2AX and complex member NHP10 (7,8). INO80 also plays an important role in transcription as it is required for proper pausing of RNA polymerase II (9).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nChromatin-remodeling ATPase INO80; DNA helicase INO80; DNA helicase-related INO80 complex homolog 1; DNA helicase-related protein INO80; hINO80; homolog of yeast INO80; INO80; INO80 complex ATPase subunit; INO80 complex homolog 1; INO80 complex subunit A; INO80 homolog (S. cerevisiae); INO80A; INOC1; KIAA1259; Putative DNA helicase INO80 complex homolog 1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 280\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799884681385,"sku":"73828S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-73828s","provider":"Iright","version":"1.0","type":"link"}