{"product_id":"cst-73858s","title":"CST,  73858S, Cre Recombinase (D7L7L) Rabbit Monoclonal Antibody (Alexa Fluor® 647 Conjugate)","description":"Monoclonal Antibody for studying cre recombinase. Validated for Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed\/Permeabilized). Highly specific and rigorously validated in-house, Cre Recombinase (D7L7L) Rabbit Monoclonal Antibody (Alexa Fluor\u003csup\u003e®\u003c\/sup\u003e 647 Conjugate) (CST #73858) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nImmunofluorescence (Frozen): 1:50 - 1:200\nImmunofluorescence (Immunocytochemistry): 1:50 - 1:100\nFlow Cytometry (Fixed\/Permeabilized): 1:200\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCre Recombinase (D7L7L) Rabbit Monoclonal Antibody (Alexa Fluor\nSpecies Reactivity: All Species Expected\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of bacteriophage-P1 Cre recombinase protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCre recombinase is a bacteriophage-P1 enzyme required for maintenance of the phage genome as a monomeric plasmid in the lysogenic state (1,2). This enzyme mediates a site-specific recombination between two 34-base-pair loxP sites. This reaction can be carried out , indicating that it does not require accessory factors (3). The Cre\/Lox system has been used for a number of and applications, including targeted gene deletions (4) and gene-specific humanized animal models (5). Resolution of the crystal structure of the Cre-Lox complex revealed that two Cre molecules interact with a single Lox site (6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\ncre; cre recombinase; cyclization recombinase; Recombinase cre\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: All\nSENSITIVITY: Transfected Only\nSource\/Isotype: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46800521920681,"sku":"73858S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-73858s","provider":"Iright","version":"1.0","type":"link"}