{"product_id":"cst-7389s","title":"CST,  7389S, CBP (D6C5) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying CBP. Validated for WB,WB,IP,IHC,IF,ChIP,ChIP. Available in 3 sizes. Highly specific and rigorously validated in-house, CBP (D6C5) Rabbit Monoclonal Antibody (CST #7389) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits.\nWestern Blotting: 1:1000\nSimple Western™: 1:50 - 1:250\nImmunoprecipitation: 1:200\nImmunohistochemistry (Paraffin): 1:50\nImmunofluorescence (Immunocytochemistry): 1:100 - 1:200\nChromatin IP: 1:50\nChromatin IP-seq: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 63794 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Chromatin IP, Chromatin IP-seq\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCBP (D6C5) Rabbit Monoclonal Antibody recognizes endogenous levels of total CBP protein. This antibody does not cross-react with p300 protein. Non-specific cytoplasmic staining was observed in cardiomyocytes by immunohistochemistry.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a recombinant protein specific to the amino terminus of human CBP protein. The epitope has been mapped to residues surrounding Ser235.\n\u003cb\u003eBackground\u003c\/b\u003e\nCBP (CREB-binding protein) and p300 are highly conserved and functionally related transcriptional co-activators that associate with transcriptional regulators and signaling molecules, integrating multiple signal transduction pathways with the transcriptional machinery (1,2). CBP\/p300 also contain histone acetyltransferase (HAT) activity, allowing them to acetylate histones and other proteins (2). Phosphorylation of p300 at Ser89 by PKC represses its transcriptional activity, and phosphorylation at the same site by AMPK disrupts the association of p300 with nuclear receptors (3,4). Ser1834 phosphorylation of p300 by Akt disrupts its association with C\/EBPβ (5). Growth factors induce phosphorylation of CBP at Ser437, which is required for CBP recruitment to the transcription complex (6). CaM kinase IV phosphorylates CBP at Ser302, which is required for CBP-dependent transcriptional activation in the CNS (7). The role of acetylation of CBP\/p300 is of particular interest (2,8). Acetylation of p300 at Lys1499 has been demonstrated to enhance its HAT activity and affect a wide variety of signaling events (9).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCBP; CREB binding protein; CREB-binding protein; CREBBP; Histone lysine acetyltransferase CREBBP; KAT3A; MKHK1; Protein-lysine acetyltransferase CREBBP; RSTS; RSTS1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 300\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799884845225,"sku":"7389S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-7389s","provider":"Iright","version":"1.0","type":"link"}