{"product_id":"cst-74139t","title":"CST,  74139T, BANK1 (F4G1Q) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying BANK1. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, BANK1 (F4G1Q) Rabbit Monoclonal Antibody (CST #74139) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:50 - 1:250\nImmunoprecipitation: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nBANK1 (F4G1Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total BANK1 protein. This antibody may detect bands of unknown origin around 50-60 kDa.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala632 of human BANK1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nB cell scaffold protein with ankyrin repeats (BANK1) is an adaptor protein that is highly expressed in B lineage but not T lineage cells (1). BANK1 is phosphorylated upon B cell antigen receptor (BCR) stimulation and functions as a scaffolding protein, promoting Lyn-mediated phosphorylation of inositol 1,4,5-triphosphate receptor (IP3R) and downstream calcium signaling (1). BANK1 also associates with B lymphoid tyrosine kinase (BLK) and phospholipase C gamma 2 (PLCg2), two additional mediators of B cell signaling (2,3). Knockout studies in mice suggest that BANK1 additionally acts as a negative regulator of CD40-mediated Akt activation (4). gene mutations are associated with several diseases, including systemic lupus erythematosus (SLE), systemic sclerosis, and rheumatoid arthritis (5-7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nB cell scaffold protein with ankyrin repeats 1; B-cell scaffold protein with ankyrin repeats; B-cell scaffold protein with ankyrin repeats 1; BANK; BANK1; FLJ20706; FLJ34204\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 105\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799886876841,"sku":"74139T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-74139t","provider":"Iright","version":"1.0","type":"link"}