{"product_id":"cst-74193s","title":"CST,  74193S, X-34","description":"Cellular Dyes for studying in the research area.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nSupplied Reagents: 1. X-34:Create a 5 mM stock solution of X-34 (200X) using the X-34 diluent. Create a 25 µM staining solution by further diluting the 5 mM stock solution using the X-34 diluent. Note: X-34 diluent (40% EtOH, pH 10) is the recommended solvent for staining. A slight precipitate may be present, but will not interfere with the product's performance. Additional Reagants (Not Supplied) 1. X-34 diluent (40% EtOH, pH 10): Combine 20 mL of EtOH in 29.5 mL of dH 2 O. Add 1 µL of 5M NaOH dropwise until the solution reaches pH 10. Bring the final volume of X-34 diluent to 50 mL with dH 2 O. 2. Differentiation buffer: 50 mM NaOH, 80% EtOH Protocol: 1. Stain slides in 25 µM X-34 staining solution for 30 min at room temperature in the dark. 2. Rinse slides three times in dH 2 O. 3. Incubate for 2 min in the differentiation buffer. 4. Rinse slides three times in dH 2 O. 5. If desired, proceed with immunostaining. 6. Mount and image.\n\u003cb\u003eStorage\u003c\/b\u003e\nStore lyophilized at 4ºC, desiccated. In lyophilized form, the product is stable for 12 months.\n\u003cb\u003eBackground\u003c\/b\u003e","brand":"CST","offers":[{"title":"Default Title","offer_id":46800734290089,"sku":"74193S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-74193s","provider":"Iright","version":"1.0","type":"link"}