{"product_id":"cst-7421s","title":"CST,  7421S, MAPKAPK-3 (D54E4) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying MAPKAPK-3. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, MAPKAPK-3 (D54E4) Rabbit Monoclonal Antibody (CST #7421) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nMAPKAPK-3 (D54E4) Rabbit Monoclonal Antibody recognizes endogenous levels of total MAPKAPK-3 protein. This antibody does not cross-react with MAPKAPK-2 or MAPKAPK-5.\nSpecies Reactivity: Human, Mouse, Rat, Monkey, Pig\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro34 of human MAPKAPK-3 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nMAPKAPK-3 has a single potential SH3-binding site in the proline-rich amino terminus, a putative ATP-binding site, 2 MAP kinase phosphorylation site motifs, and a putative nuclear localization signal. It shares 72% nucleotide and 75% amino acid identity with MAPKAPK-2 (1). MAPKAPK-3 has been shown to be activated by growth inducers and stress stimulation of cells. studies have demonstrated that Erk, p38 MAP kinase, and Jun amino-terminal kinase are able to phosphorylate and activate MAPKAPK-3, which suggested a role for this kinase as an integrative element of signaling in both mitogen and stress responses (2). MAPKAPK-3 was reported to interact with, phosphorylate, and repress the activity of E47, which is a basic helix-loop-helix transcription factor involved in the regulation of tissue-specific gene expression and cell differentiation (3). MAPKAPK-3 may also support luteal maturation through the phosphorylation and activation of the nuclear transcription factor CREB (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n3pK; Chromosome 3p kinase; MAP kinase-activated protein kinase 3; MAPK activated protein kinase 3; MAPK-activated protein kinase 3; MAPK3; MAPKAP kinase 3; MAPKAP-K3; MAPKAP3; MAPKAPK-3; MAPKAPK3; MDPT3; mitogen-activated protein kinase-activated protein kinase 3; MK-3; MK3\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk Pg\nSENSITIVITY: Endogenous\nMW (kDa): 42\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799887204521,"sku":"7421S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-7421s","provider":"Iright","version":"1.0","type":"link"}