{"product_id":"cst-74223s","title":"CST,  74223S, RUFY3 (E6H5Z) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying RUFY3. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, RUFY3 (E6H5Z) Rabbit Monoclonal Antibody (CST #74223) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nRUFY3 (E6H5Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total RUFY3 protein. This antibody detects multiple RUFY3 isoforms reported to be expressed by alternative splicing, including human RUFY3 isoform 1 and 2.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His58 of human RUFY3 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nRUN and FYVE domain-containing protein 3 (RUFY3) is an actin-binding protein that is enriched in the brain. RUFY3 was identified as a protein that is upregulated during neuronal polarization, which is important for proper neuron functioning (1). RUFY3 likely functions in regulating neuronal polarization by interacting with the neuronal cytoskeleton via actin filament-binding proteins, such as Fascin (2). RUFY3 undergoes various forms of posttranslational modifications, including CASP3-dependent cleavage and phosphorylation, which may modulate selective synaptic and axonal degeneration during brain development and neurodegenerative disease (3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nKIAA0871; Protein RUFY3; rap2 interacting protein x; Rap2-interacting protein x; RIPx; RUFY3; RUN and FYVE domain containing 3; RUN and FYVE domain-containing protein 3; Singar; SINGAR1; Single axon-regulated protein; single axon-related 1; ZFYVE30\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 53, 54\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799887335593,"sku":"74223S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-74223s","provider":"Iright","version":"1.0","type":"link"}