{"product_id":"cst-74244t","title":"CST,  74244T, Estrogen Receptor alpha (D6R2W) Rabbit Monoclonal Antibody (PE Conjugate)","description":"Monoclonal Antibody for studying ER-alpha. Validated for Flow Cytometry (Fixed\/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, Estrogen Receptor alpha (D6R2W) Rabbit Monoclonal Antibody (PE Conjugate) (CST #74244) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFlow Cytometry (Fixed\/Permeabilized): 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg\/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nEstrogen Receptor alpha (D6R2W) Rabbit Monoclonal Antibody (PE Conjugate) recognizes endogenous levels of total estrogen receptor α protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human estrogen receptor α protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nEstrogen receptor α (ERα), a member of the steroid receptor superfamily, contains highly conserved DNA binding and ligand binding domains (1). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ERα regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery (2). Phosphorylation at multiple sites provides an important mechanism to regulate ERα activity (3-5). Ser104, 106, 118, and 167 are located in the amino-terminal transcription activation function domain AF-1, and phosphorylation of these serine residues plays an important role in regulating ERα activity. Ser118 may be the substrate of the transcription regulatory kinase CDK7 (5). Ser167 may be phosphorylated by p90RSK and Akt (4,6). According to the research literature, phosphorylation at Ser167 may confer tamoxifen resistance in breast cancer patients (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nDKFZp686N23123; E2 receptor alpha; ER; ER-alpha; Era; ESR; ESR1; ESRA; Estradiol receptor; estrogen nuclear receptor alpha; Estrogen receptor; estrogen receptor 1; estrogen receptor alpha delta 3; estrogen receptor alpha delta 4; estrogen receptor alpha delta 4 +49 isoform; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; ESTRR; NR3A1; Nuclear receptor subfamily 3 group A member 1; oestrogen receptor alpha\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800522707113,"sku":"74244T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-74244t","provider":"Iright","version":"1.0","type":"link"}