{"product_id":"cst-74471s","title":"CST,  74471S, SARS-CoV-1 Membrane Protein Antibody","description":"Polyclonal Antibody for studying Membrane Protein SARS-CoV. Validated for Western Blotting. Highly specific and rigorously validated in-house, SARS-CoV-1 Membrane Protein Antibody (CST #74471) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nSARS-CoV-1 Membrane Protein Antibody recognizes transfected levels of total SARS-CoV-1 membrane protein. This antibody does not cross-react with SARS-CoV-2 or MERS-CoV membrane proteins.\nSpecies Reactivity: Virus\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly211 of SARS-CoV-1 membrane protein. Antibodies are purified by peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe cause of the SARS epidemic in 2003 was a novel pathogenic coronavirus, originally termed SARS-CoV (severe acute respiratory syndrome coronavirus) (1). Following the 2019 emergence of SARS-CoV-2 and the COVID-19 pandemic, SARS-CoV is now often referred to as SARS-CoV-1. SARS-CoV-1 is a member of the Coronaviridae family of single-stranded RNA viruses (2). The genome of SARS-CoV-1 is relatively large among viruses (~30 kDa); it encodes four key structural proteins (spike, envelope, membrane, and nucleocapsid) that make up the virion particle, in addition to a variety of smaller open reading frames (ORFs) that encode the accessory proteins required for viral replication (3). The SARS-CoV-1 membrane protein (M) is the most abundant protein in the viral envelope; in addition to its structural role, it plays an important role in virus-host interactions and the viral lifecycle (4). Notably, studies have shown that membrane proteins from both SARS-CoV-1 and SARS-CoV-2 may induce apoptosis in host cells, suggesting one mechanism by which SARS coronaviruses cause tissue damage upon infection (5,6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nE1 glycoprotein; M protein; Matrix glycoprotein; matrix protein; Membrane glycoprotein; membrane glycoprotein M; Membrane protein; SARS-CoV membrane protein; sars5; VME1_CVHSA\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: Vir\nSENSITIVITY: Transfected Only\nMW (kDa): 20-30\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46800244834473,"sku":"74471S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-74471s","provider":"Iright","version":"1.0","type":"link"}