{"product_id":"cst-74683t","title":"CST,  74683T, MTAP (E5R1I) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying MTAP. Validated for Western Blotting,IHC Leica Bond,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, MTAP (E5R1I) Rabbit Monoclonal Antibody (CST #74683) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nIHC Leica Bond: 1:400 - 1:1600\nImmunohistochemistry (Paraffin): 1:50 - 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 73819 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nMTAP (E5R1I) Rabbit Monoclonal Antibody recognizes endogenous levels of total human MTAP protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human MTAP protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nMTAP is an enzyme that is essential for the salvage pathway for both adenine and methionine synthesis. MTAP catalyzes the cleavage of 5'-methylthioadenosine into adenine and 5-methylthio-D-ribose-1-phosphate. Adenine is then used to generate AMP whereas 5-methylthio-D-ribose-1-phosphate is converted into methionine (1,2). MTAP is expressed in all normal cells and tissues, although frequently lost in different human tumors, including pancreatic adenocarcinoma, neuroendocrine tumors, non-small cell lung carcinoma, and breast carcinoma. MTAP is usually codeleted with p16 (cdkN2a\/ARF) (3-5). MTAP overexpression in breast cancer cells inhibits their ability to form colonies in soft agar, thereby implicating its function as a tumor suppressor (6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n5'-methylthioadenosine phosphorylase; BDMF; c86fus; DMSFH; DMSMFH; epididymis luminal protein 249; epididymis secretory sperm binding protein; HEL-249; LGMBF; MeSAdo phosphorylase; methylthioadenosine phosphorylase; MSAP; MTA phosphorylase; MTAP; MTAPase; S-methyl-5'-thioadenosine phosphorylase\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 29\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799888810153,"sku":"74683T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-74683t","provider":"Iright","version":"1.0","type":"link"}