{"product_id":"cst-74731s","title":"CST,  74731S, Puma (E2P7G) Rabbit Monoclonal Antibody (Biotinylated)","description":"Monoclonal Antibody for studying PUMA. Validated for Western Blotting. Highly specific and rigorously validated in-house, Puma (E2P7G) Rabbit Monoclonal Antibody (Biotinylated) (CST #74731) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 136 mM NaCl, 2.6 mM KCI, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg\/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPuma (E2P7G) Rabbit Monoclonal Antibody (Biotinylated) recognizes endogenous levels of total Puma protein.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly28 of human Puma protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nPuma (p53 upregulated modulator of apoptosis) is a \"BH3-only\" Bcl-2 family member originally identified in differential gene expression studies as a p53-inducible gene (1,2). The \"BH3-only\" family members include Bad, Bid, Bik, Hrk, Bim, and Noxa, all of which contain a BH3 domain but lack other conserved domains, BH1 and BH2, and generally promote apoptosis by binding to and antagonizing anti-apoptotic Bcl-2 family members through BH3 domain interactions (3). Two BH3-containing proteins are produced from the gene, Puma-α and Puma-β, both of which are induced by p53, bind Bcl-2 and Bcl-xL, localize to the mitochondria, and promote cytochrome c release and apoptosis (1,2). Puma plays a critical role in the p53 tumor suppressor pathway. Targeted disruption of the gene impairs p53-mediated apoptosis and tumor suppression (4-7). Puma knockout mice show defects from multiple apoptotic stimuli, including ionizing irradiation, deregulated c-Myc expression, and cytokine withdrawal (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nBBC3; Bcl-2-binding component 3; Bcl-2-binding component 3, isoforms 1\/2; BCL2 binding component 3; FLJ42994; JFY-1; JFY1; p53 up-regulated modulator of apoptosis; p53-upregulated modulator of apoptosis; PUMA\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nMW (kDa): 23\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800523428009,"sku":"74731S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-74731s","provider":"Iright","version":"1.0","type":"link"}