{"product_id":"cst-74778s","title":"CST,  74778S, Phospho-Rad50 (Ser635) Antibody","description":"Polyclonal Antibody for studying Rad50 (Ser635) phosphate. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Highly specific and rigorously validated in-house, Phospho-Rad50 (Ser635) Antibody (CST #74778) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-Rad50 (Ser635) Antibody recognizes endogenous levels of Rad50 protein only when phosphorylated at Ser635.\nSpecies Reactivity: Human, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser635 of human Rad50 protein. Antibodies are purified by peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe DNA repair protein Rad50 is a member of the structural maintenance of chromosomes (SMC) family and plays an important role in cell cycle checkpoint signaling and double-strand break repair in response to DNA damage (1-4). Rad50 forms a complex with Mre11 and NBS1 that becomes activated in response to DNA damage (3). In normal human cells, the MRN complex acts to tether linear DNA molecules, providing a flexible link between DNA ends (1). Genomic instability and cancer have been shown to develop in cells with genetic mutations affecting the proteins in the MRN complex (2). ATM-dependent phosphorylation of Rad50 at Ser635 in response to DNA damage is important in regulating downstream signaling, DNA repair, and checkpoint control (5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nDNA repair protein RAD50; hRAD50; NBSLD; RAD50; RAD50 double strand break repair protein; RAD50 homolog (S. cerevisiae); RAD50 homolog, double strand break repair protein; RAD50-2; RAD502\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H R\nSENSITIVITY: Endogenous\nMW (kDa): 153\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799889301673,"sku":"74778S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-74778s","provider":"Iright","version":"1.0","type":"link"}