{"product_id":"cst-74829t","title":"CST,  74829T, Histone H3 (K27M Mutant Specific) (D3B5T) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying H3F3A (Lys27Met) mutation. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed\/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, Histone H3 (K27M Mutant Specific) (D3B5T) Rabbit Monoclonal Antibody (CST #74829) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunofluorescence (Immunocytochemistry): 1:1600\nFlow Cytometry (Fixed\/Permeabilized): 1:400 - 1:1600\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 10376 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nHistone H3 (K27M Mutant Specific) (D3B5T) Rabbit Monoclonal Antibody recognizes endogenous levels of K27M mutant histone H3.1, H3.2, and H3.3 proteins. This antibody may cross-react with wild-type histone H3.1, 3.2, and 3.3 when used at a high concentration. Careful titration of this antibody may be required to obtain optimal specificity.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to K27M mutant sequence of human histone H3.3 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nDiffuse intrinsic pontine glioma (DIPG) is an aggressive brainstem astrocyte tumor arising mostly in children, leading to a long-term survival rate of less than 10%. Multiple whole-genome sequencing studies of DIPG patients identified commonly occurring mutations in the H3F3A gene encoding histone H3.3. One of these mutations, a lysine to methionine amino acid substitution (K27M), is found in up to 78% of DIPGs and 22% of non-brainstem pediatric gliomas (1-3). This mutation is associated with poor prognosis, with a mean survival time of 0.73 years for patients with the K27M mutation versus 4.6 years for patients without the mutation (1-3). Expression of the K27M mutant histone H3 is accompanied by a dramatic reduction in the levels of polycomb repressive complex 2 (PRC2)-mediated trimethylation of histone H3, changes in the distribution of PRC2 on the genome, and altered expression of genes associated with various cancer pathways (4-6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nH3 histone family member 3A; H3 histone, family 3A; H3 histone, family 3B (H3.3B); H3-3A; H3-3B; H3.3 histone A; H3.3A; H3.3B; H33; H3F3; H3F3A; H3F3B; Histone H3.3\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nMW (kDa): 17\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799889629353,"sku":"74829T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-74829t","provider":"Iright","version":"1.0","type":"link"}