{"product_id":"cst-75030t","title":"CST,  75030T, p75NTR (E7U5R) Mouse Monoclonal Antibody","description":"Monoclonal Antibody for studying p75NTR. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, p75NTR (E7U5R) Mouse Monoclonal Antibody (CST #75030) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\np75NTR (E7U5R) Mouse Monoclonal Antibody recognizes endogenous levels of total p75NTR protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro370 of human p75NTR protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe p75 neurotrophin receptor (p75NTR), a member of the TNF receptor superfamily, is distinguished by multiple cysteine-rich ligand-binding domains, a single transmembrane sequence, and a noncatalytic cytoplasmic domain (1). p75NTR displays paradoxical functions when acting alone or with other receptor proteins. Working in concert with Trk receptors, p75NTR recognizes neurotrophins and transmits trophic signals into the cell. Both p75NTR and TrkA are required to activate PI3K-Akt signaling, while TrkA can individually activate the MAP kinase pathway. In contrast, p75NTR, possibly through JNK, ensures appropriate apoptosis of injured neurons and improperly targeted neonatal neurons (2). The p75NTR protein undergoes sequential cleavage similar to APP and Notch. First, α-secretase removes the p75NTR ectodomain, eliminating ligand-mediated signaling. At this point, the membrane-tethered cleavage product can still fine-tune Trk-mediated trophic actions. γ-secretase cleaves within the transmembrane domain to liberate the cytoplasmic tail from its membrane anchor and allow the p75NTR intracellular domain to translocate to the nucleus (3,4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCD271; Gp80-LNGFR; low affinity nerve growth factor receptor; Low affinity neurotrophin receptor p75NTR; Low-affinity nerve growth factor receptor; nerve growth factor receptor; nerve growth factor receptor (TNFR superfamily, member 16); NGF receptor; NGFR; p75 ICD; p75(NTR); p75NTR; TNFR superfamily, member 16; TNFRSF16; TNR16; Tumor necrosis factor receptor superfamily member 16\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 75\nSource\/Isotype: Mouse IgG2b kappa","brand":"CST","offers":[{"title":"Default Title","offer_id":46799890546857,"sku":"75030T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-75030t","provider":"Iright","version":"1.0","type":"link"}