{"product_id":"cst-75148t","title":"CST,  75148T, Phospho-PKD\/PKC mu (Ser910) (E7M8C) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying PKD1\/PKCmu (Ser910) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-PKD\/PKC mu (Ser910) (E7M8C) Rabbit Monoclonal Antibody (CST #75148) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-PKD\/PKC mu (Ser910) (E7M8C) Rabbit Monoclonal Antibody detects endogenous levels of PKD\/PKCμ protein only when phosphorylated at Ser910.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser910 of human PKD\/PKCμ protein. This site is homologous to Ser916 in mouse sequence.\n\u003cb\u003eBackground\u003c\/b\u003e\nActivation of PKC is one of the earliest events in a cascade leading to a variety of cellular responses, such as secretion, gene expression, proliferation, and muscle contraction (1,2). Protein kinase D (PKD), also called PKCμ, is a serine\/threonine kinase whose activation is dependent on the phosphorylation of two activation loop sites, Ser744 and Ser748, via a PKC-dependent signaling pathway (3-5). In addition to the two activation loop sites, the carboxy-terminal Ser916 has been identified as an autophosphorylation site for PKD\/PKCμ. Phosphorylation at Ser916 correlates with PKD\/PKCμ catalytic activity (6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCHDED; KPCD1; nPKC-D1; nPKC-mu; PKC-MU; PKCM; PKD; PKD1; PRKCM; PRKD1; Protein kinase C mu type; protein kinase C, mu; Protein kinase D; protein kinase D1; Serine\/threonine-protein kinase D1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nMW (kDa): 115\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799890940073,"sku":"75148T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-75148t","provider":"Iright","version":"1.0","type":"link"}