{"product_id":"cst-75628s","title":"CST,  75628S, Phospho-PFAS (Thr619) (F1L7L) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying PFAS (Thr619) phosphate. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-PFAS (Thr619) (F1L7L) Rabbit Monoclonal Antibody (CST #75628) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-PFAS (Thr619) (F1L7L) Rabbit Monoclonal Antibody recognizes endogenous levels of PFAS protein only when phosphorylated at Thr619.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr619 of human PFAS protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nPhosphoribosylformylglycinamidine synthase (PFAS) is a metabolic enzyme (deamidase) associated with a protein complex known as the \"purinosome,\" which is involved in purine synthesis (1). Research studies have shown that activation of the MAPK\/ERK pathway leads to downstream activation of PFAS. Specifically, it was shown that PFAS is a substrate of ERK2, which phosphorylates PFAS at Thr619; activation of PFAS results in the generation of purine intermediates required for cell and tumor growth (2). It has also been reported that both PFAS and viral PFAS-like proteins can activate RIG-I pattern recognition receptors, implicating PFAS-mediated deamidation in the innate immune response to viral infection (3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nFGAM synthase; FGAMS; FGAR amidotransferase; FGAR-AT; FGARAT; Formylglycinamide ribonucleotide amidotransferase; Formylglycinamide ribotide amidotransferase; Formylglycinamide ribotide synthetase; GATD8; IKZF1\/PFAS fusion; KIAA0361; PFAS; Phosphoribosylformylglycinamidine synthase; PUR4; PURL\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 145\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799892840617,"sku":"75628S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-75628s","provider":"Iright","version":"1.0","type":"link"}