{"product_id":"cst-75753s","title":"CST,  75753S, ZO-1 (6B6E4) Rat Monoclonal Antibody (Alexa Fluor® 488 Conjugate)","description":"Monoclonal Antibody for studying ZO-1. Validated for Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, ZO-1 (6B6E4) Rat Monoclonal Antibody (Alexa Fluor\u003csup\u003e®\u003c\/sup\u003e 488 Conjugate) (CST #75753) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nImmunofluorescence (Immunocytochemistry): 1:50 - 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg\/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nZO-1 (6B6E4) Rat Monoclonal Antibody (Alexa Fluor\nSpecies Reactivity: Human, Dog\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a recombinant protein corresponding to sequences within the PDZ1 and PDZ2 domains of human ZO-1.\n\u003cb\u003eBackground\u003c\/b\u003e\nTight junctions, or zona occludens (ZO), form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and the basolateral cell surfaces (reviewed in 1). ZO-1, -2, and -3 (also known as TJP1, 2, and 3) are peripheral membrane adaptor proteins that link junctional transmembrane proteins, such as occludin and claudin, to the actin cytoskeleton (reviewed in 2). ZO-1 and ZO-2 are required for tight junction formation and function (3,4). In subconfluent proliferating cells, ZO-1 and ZO-2 have been shown to colocalize to the nucleus and play a role in transcriptional regulation, possibly through facilitating nuclear import\/export of transcriptional regulators (5-7). The gene is transcribed from two promoters, generating the ZO-2A and ZO-2C isoforms. ZO-2C lacks a 23 amino acid amino-terminal sequence found in other ZO-2 isoforms. While both isoforms appear to be widely expressed, abnormal regulation of the gene may be correlated with development of ductal cancer (8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nDKFZp686M05161; MGC133289; Tight junction protein 1; tight junction protein 1 (zona occludens 1); Tight junction protein ZO-1; TJP1; ZO-1; ZO1; zona occludens 1; Zona occludens protein 1; zonula occludens 1 protein; Zonula occludens protein 1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H Dg\nSENSITIVITY: Endogenous\nSource\/Isotype: Rat IgG2a","brand":"CST","offers":[{"title":"Default Title","offer_id":46800525426857,"sku":"75753S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-75753s","provider":"Iright","version":"1.0","type":"link"}