{"product_id":"cst-75959s","title":"CST,  75959S, Phospho-Atg101 (Ser11) (E5Z5A) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying ATG101 (Ser11) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Atg101 (Ser11) (E5Z5A) Rabbit Monoclonal Antibody (CST #75959) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-Atg101 (Ser11) (E5Z5A) Rabbit Monoclonal Antibody recognizes transfected levels of Atg101 protein only when phosphorylated at Ser11.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser11 of human Atg101 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nAtg101 was discovered as a binding protein for Atg13, a component of the ULK1 serine-threonine kinase complex required for autophagy (1-3). Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (4,5). It is generally activated by conditions of nutrient deprivation, but is also associated with a number of physiological processes, including development, differentiation, neurodegeneration, infection, and cancer (6). The molecular machinery of autophagy was largely discovered in yeast and is directed by a number of autophagy-related (Atg) genes. The ULK1 complex includes both Atg13 and FIP200 and is required for starvation-induced autophagy (7-9). Interaction between Atg101 and Atg13 can be important for the stability and basal phosphorylation of Atg13 and ULK1 (1,2). Atg101 has been reported to be phosphorylated at Ser11 by ULK1 (10).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nATG101; Atg13-interacting protein; ATGA1; autophagy related 101; Autophagy-related protein 101; C12orf44; chromosome 12 open reading frame 44; FLJ11773\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Transfected Only\nMW (kDa): 25\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799893725353,"sku":"75959S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-75959s","provider":"Iright","version":"1.0","type":"link"}