{"product_id":"cst-76103s","title":"CST,  76103S, TMEM119 (E3E1O) Feline Chimeric Monoclonal Antibody","description":"Monoclonal Antibody for studying TMEM119. Validated for Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, TMEM119 (E3E1O) Feline Chimeric Monoclonal Antibody (CST #76103) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nImmunofluorescence (Frozen): 1:50 - 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Immunofluorescence (Frozen)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nTMEM119 (E3E1O) Feline Chimeric Monoclonal Antibody recognizes endogenous levels of total TMEM119 protein.\nSpecies Reactivity: Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nThis recombinant chimeric antibody is engineered from TMEM119 (E3E1O) Rabbit mAb #90840 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody, but contains a feline-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies. The parent antibody, TMEM119 (E3E1O) Rabbit mAb #90840, is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human TMEM119 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nTransmembrane protein 119 (TMEM119) is a cell-surface protein of unknown function, expressed exclusively by the microglia subset of myeloid and neural cells (1). Iba1+ microglia with both ramified and amoeboid morphologies express TMEM119, while Iba1+ macrophages are TMEM119 negative (2). and other homeostatic genes have been shown to be downregulated in disease-associated microglia (DAM). DAM are conserved in human and mouse and have been identified in Alzheimer's disease (AD), amyotrophic lateral sclerosis (ALS), and 5XFAD and APP\/PS1 mouse models (3,4). This protein's specificity as a microglia marker has proven itself important for labeling microglia in healthy tissue as well as deciphering them from infiltrating macrophages and other cell types in neurodegenerative disease models (1,2).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nOBIF; Osteoblast induction factor; TM119; TMEM119; Transmembrane protein 119\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M\nSENSITIVITY: Endogenous\nSource\/Isotype: Feline chimeric IgG1","brand":"CST","offers":[{"title":"Default Title","offer_id":46800299360425,"sku":"76103S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-76103s","provider":"Iright","version":"1.0","type":"link"}