{"product_id":"cst-76194s","title":"CST,  76194S, FAT10 (D1Q3Y) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying FAT10. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, FAT10 (D1Q3Y) Rabbit Monoclonal Antibody (CST #76194) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nFAT10 (D1Q3Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total FAT10 protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human FAT10 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nHLA-F adjacent transcript 10 (FAT10\/Ubiquitin D) belongs to the ubiquitin-like modifier (Ubl) family of proteins. The 18 kDa FAT10 protein contains two tandem Ubl domains that are oriented in a head-to-tail fashion and a free C-terminal di-glycine motif, which is available for isopeptide bond formation with target proteins via an E1-E2-E3 enzymatic cascade (1). Indeed, FAT10 provides a ubiquitin-independent signal for proteasomal degradation (2). Research studies have demonstrated that FAT10 expression is enriched in lymphoid organs and that its expression is transiently upregulated via the NF-kB pathway in response to pro-inflammatory cytokines such as TNFα and IFNγ (1,3-5). In solid tumors that possess inflammatory microenviroments, research studies have shown that FAT10 is overexpressed and may serve as a biomarker for inflamed tumors (3,4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nDiubiquitin; FAT10; GABBR1; UBD; UBD-3; Ubiquitin D; Ubiquitin-like protein FAT10\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 18\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799895232681,"sku":"76194S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-76194s","provider":"Iright","version":"1.0","type":"link"}