{"product_id":"cst-76439s","title":"CST,  76439S, Gasdermin B Antibody","description":"Polyclonal Antibody for studying Gasdermin B. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Gasdermin B Antibody (CST #76439) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nGasdermin B Antibody recognizes endogenous levels of total Gasdermin B protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu96 of human Gasdermin B protein. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe gasdermin family, which includes GSDMA, GSDMB, GSDMC, GSDMD, and GSDME, has been shown to play a role in inflammation and cell death. Gasdermin D has been reported to have a critical role as a downstream effector of pyroptosis (1,2). Pyroptosis is a lytic type of cell death triggered by inflammasomes, multiprotein complexes assembled in response to pathogen-associated molecular patterns (PAMPs) or danger-associated molecular patterns (DAMPs) that result in the activation of caspase-1 and subsequent cleavage of pro-inflammatory cytokines IL-1β and IL-18 (3). Gasdermin D was identified by two independent groups as a substrate of inflammatory caspases, caspase-1 and caspase-11\/4\/5, producing two fragments: GSDMD-N and GSDMD-C. Cleavage results in release of an intramolecular inhibitory interaction between the N- and C-terminal domains, allowing the N-terminal fragment GSDMD-N to initiate pyroptosis through the formation of pores on the plasma membrane (4-7). Gasdermin B (GSDMB) has been reported to be upregulated in several tumor types, and in breast cancer has been associated with metastasis and poor prognosis (8,9). In addition Gasdermin B has been associated with immune disorders including asthma (10,11). Gasdermin B expression has also be found in the lung epithelium associated with asthma. Gasdermin B can also have a role in pyroptosis as it was found to activate caspase-4 and promote Gasdermin D cleavage (12).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\ngasdermin B; Gasdermin-B; Gasdermin-like protein; gasderminB-1; GSDMB; GSDMB-1; GSDML; PP4052; PRO2521\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 47\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799896772777,"sku":"76439S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-76439s","provider":"Iright","version":"1.0","type":"link"}