{"product_id":"cst-76684t","title":"CST,  76684T, IRF-9 (D2T8M) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying IRF-9. Validated for WB,IP,IF,ChIP. Available in 2 sizes. Highly specific and rigorously validated in-house, IRF-9 (D2T8M) Rabbit Monoclonal Antibody (CST #76684) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits.\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nImmunofluorescence (Immunocytochemistry): 1:3200\nChromatin IP: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Chromatin IP\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nIRF-9 (D2T8M) Rabbit Monoclonal Antibody recognizes endogenous levels of total IRF-9 protein. This antibody also cross-reacts with an unidentified protein of 95 kDa. Non-specific nucleolar background has been observed by immunofluorescence in some cultured cell lines.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein specific to human IRF-9 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nInterferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak\/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, IRF-9\/ISGF3γ, IRF-3, IRF-4 (Pip\/LSIRF\/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8\/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E) (2). In response to type I interferons (e.g., IFN- α, IFN-β), interferon regulatory factor 9 (IRF-9, ISGF3γ) assembles into a protein complex with Stat1 and Stat2 and translocates to the nucleus where it induces transcription of interferon response genes by binding to ISREs (3,4). Expression of IRF-9 is also induced by type I interferons (5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nIFN-alpha-responsive transcription factor subunit; Interferon regulatory factor 9; Interferon-stimulated gene factor 3 gamma; interferon-stimulated transcription factor 3, gamma (48kD); interferon-stimulated transcription factor 3, gamma 48kDa; IRF-9; IRF9; ISGF-3 gamma; ISGF3; ISGF3 p48 subunit; ISGF3G; p48; Transcriptional regulator ISGF3 subunit gamma\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 48\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799897886889,"sku":"76684T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-76684t","provider":"Iright","version":"1.0","type":"link"}