{"product_id":"cst-76851s","title":"CST,  76851S, NADK Isoform 3 (F5E2T) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying NADK iso3. Validated for Western Blotting. Highly specific and rigorously validated in-house, NADK Isoform 3 (F5E2T) Rabbit Monoclonal Antibody (CST #76851) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nNADK Isoform 3 (F5E2T) Rabbit Monoclonal Antibody recognizes endogenous levels of total NADK isoform 3 protein (UniProt #O95544-3). This antibody does not cross-react with other NADK isoforms. This antibody cross-reacts with a 125 kDa protein of unknown identity in some cell lines.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile49 of human NADK isoform 3 protein (Uniprot #O95544-3).\n\u003cb\u003eBackground\u003c\/b\u003e\nNADK, also known as NAD kinase, is a cytoplasmic protein responsible for maintaining the pool of available NADP and NADPH within the cell (1). Using ATP as a phosphate donor, NADK catalyzes the phosphorylation of NAD to NADP . This molecule is then reduced to NADPH and utilized in various metabolic and biosynthetic pathways (2). NADK has been suggested to play a role in glucose metabolism due to the effect NADPH production has on both the insulin secretion and survival of pancreatic β-cells (3). NADPH has a vital role in protecting cells from oxidative stress through its neutralizing effect on reactive oxygen species (ROS), which also accumulate during cell growth (2-4). Along with the p53 tumor suppression protein, NADK has been a suggested target in cancer therapy due to its link to NADPH production and its resulting protective role on growing and proliferating cells (2). Cytoplasmic NADK isoform 3 is transcribed from a unique start site compared to isoforms 1 and 2, and lacks the N-terminal, Akt-dependent, activating phosphorylation sites of Ser44, Ser46, and Ser48 found in the other isoforms (5). Cells engineered to exclusively express NADK isoform 3 exhibit constitutively increased NAD kinase activity, insensitivity to AKT agonists, and increased synthesis of NADP (5). NADK isoform 3 is uniquely upregulated in metastatic nodules of a PDX model of melanoma (6). Isoform 3 expression is driven by transcription factor NRF2 in response to oxidative stress, allowing melanoma cells to survive both oxidative insult and metastatic cascade by increasing NADP synthesis (6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\ndJ283E3.1; NAD kinase\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 52\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799898542249,"sku":"76851S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-76851s","provider":"Iright","version":"1.0","type":"link"}