{"product_id":"cst-77055s","title":"CST,  77055S, cdc2 Antibody","description":"Polyclonal Antibody for studying Cdc2. Validated for Western Blotting. Highly specific and rigorously validated in-house, cdc2 Antibody (CST #77055) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\ncdc2 Antibody detects endogenous levels of total cdc2 protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp289 of human cdc2. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe entry of eukaryotic cells into mitosis is regulated by cdc2 kinase activation, a process controlled at several steps including cyclin binding and phosphorylation of cdc2 at Thr161 (1). However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of cdc2 at Thr14 and Tyr15 (2). Phosphorylation at Thr14 and Tyr15, resulting in inhibition of cdc2, can be carried out by Wee1 and Myt1 protein kinases (3,4). The cdc25 phosphatase may be responsible for removal of phosphates at Thr14 and Tyr15 and subsequent activation of cdc2 (1,5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCDC2; CDC28A; CDK1; CDKN1; cell cycle controller CDC2; Cell division control protein 2 homolog; cell division cycle 2; cell division cycle 2, G1 to S and G2 to M; Cell division protein kinase 1; cyclin dependent kinase 1; Cyclin-dependent kinase 1; DKFZp686L20222; MGC111195; p34 protein kinase; P34CDC2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 34\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799899558057,"sku":"77055S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-77055s","provider":"Iright","version":"1.0","type":"link"}