{"product_id":"cst-77510t","title":"CST,  77510T, IRG1 (D6H2Y) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying IRG1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, IRG1 (D6H2Y) Rabbit Monoclonal Antibody (CST #77510) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nIRG1 (D6H2Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total IRG1 protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly460 of human IRG1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nIRG1 (Immune-responsive gene 1) is one of the most up-regulated genes in macrophages under proinflammatory conditions (1). It is also highly expressed in the pregnant uterus during implantation (2,3). IRG1 is a cis-aconitate decarboxylase that produces itaconic acid by decarboxylating cis-aconitic acid, an intermediate of the tricarboxylic acid cycle (4). Itaconic acid is an endogenous inhibitor of succinate dehydrogenase, linking macrophage metabolic rewiring and regulation of inflammation (5,6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nACOD1; Aconitate decarboxylase; Aconitate decarboxylase 1; CAD; Cis-aconitate decarboxylase; Cis-aconitic acid decarboxylase; Immune-responsive gene 1 protein; Immune-responsive gene 1 protein homolog; immunoresponsive 1 homolog; immunoresponsive 1 homolog (mouse); IRG1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 53\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799901393065,"sku":"77510T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-77510t","provider":"Iright","version":"1.0","type":"link"}