{"product_id":"cst-77552s","title":"CST,  77552S, CUT\u0026Tag Assay Kit","description":"CUT\u0026amp;Tag Kit for studying in the research area.\n\n\u003cb\u003eStorage\u003c\/b\u003e\nAll components in this kit are stable for 6 months when stored at the recommended temperature.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: CUT\u0026amp;Tag\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nThe CUT\u0026amp;Tag Assay Kit can be utilized with any CUT\u0026amp;Tag-validated antibody to detect endogenous levels of protein-DNA interactions and histone modifications in mammalian cells (see Figures 1-3). One CUT\u0026amp;Tag reaction can use between 5,000 to 250,000 cells or 1 to 5 mg of tissue of starting material (see Figures 5-7). This kit is compatible with multiple species of antibodies, including rabbit and mouse (see Figure 4). The positive control Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit Monoclonal Antibody #9751 detects multiple species of tri-methyl histone H3 Lys4 protein, including human, mouse, rat, and monkey.\n\u003cb\u003eBackground\u003c\/b\u003e\nSimilar to Cleavage Under Targets and Release Using Nuclease (CUT\u0026amp;RUN), Cleavage Under Targets and Tagmentation (CUT\u0026amp;Tag) is a powerful technique used for probing protein-DNA interactions within the natural chromatin context of the cell (1-3). CUT\u0026amp;Tag has many of the same advantages as the CUT\u0026amp;RUN assay in that it provides a rapid, robust, and true low cell number protocol for detection of protein-DNA interactions in the cell. In addition, the CUT\u0026amp;Tag assay adds an adaptor DNA ligation step carried out by the pAG-Tn5 enzyme, in which an adaptor DNA is ligated directly to antibody-targeted chromatin DNA fragments in the cell. As a result, subsequent DNA library preparation is much faster and easier than library preparation following the CUT\u0026amp;RUN assay, free from DNA end repair, A-tailing, and adaptor ligation . CUT\u0026amp;Tag works very well for analyzing histone modifications, in addition to mapping some transcription factor and cofactor binding.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nC\u0026amp;T; CnT; CUT \u0026amp; Tag; cut and tag; CUT and Tag; CUT\u0026amp;Tag; cutandtag; histone modification","brand":"CST","offers":[{"title":"Default Title","offer_id":46800806019241,"sku":"77552S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-77552s","provider":"Iright","version":"1.0","type":"link"}