{"product_id":"cst-77568s","title":"CST,  77568S, CARD9 (E4N1E) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying CARD9 mouse. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, CARD9 (E4N1E) Rabbit Monoclonal Antibody (CST #77568) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCARD9 (E4N1E) Rabbit Monoclonal Antibody recognizes endogenous levels of total CARD9 protein.\nSpecies Reactivity: Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His478 of mouse CARD9 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCARD9 is a caspase recruitment domain (CARD)-containing adaptor protein expressed by myeloid cells (1,2). It is required for antifungal immunity downstream of pathogen detection by C-type lectin receptors (CLRs) such as Dectin-1 (3,4). Recognition of carbohydrates on fungal cell walls by CLRs leads to activation of the tyrosine kinase Syk, followed by activation of PKCδ (5,6). PKCδ phosphorylates CARD9, enabling the assembly of a complex containing CARD9 and Bcl10 (6). This complex activates NF-κB, resulting in upregulation of inflammatory cytokines important for initiation of adaptive immunity (3,4,6,7). CARD9 was also shown to be important for the induction of IL-1β, downstream of the viral nucleic acid sensor RIG-I, as well as for the generation of reactive oxygen species important for bacterial killing by macrophages (2,8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCard9; Caspase recruitment domain family, member 9; Caspase recruitment domain-containing protein 9; Gm782; OTTMUSP00000013722\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M\nSENSITIVITY: Endogenous\nMW (kDa): 64\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800123691177,"sku":"77568S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-77568s","provider":"Iright","version":"1.0","type":"link"}