{"product_id":"cst-77584w2","title":"CST,  77584W2, NK1.1\/CD161 (E6Y9G) Rabbit Monoclonal Antibody (SignalFlex™ Pacific Blue™ Conjugate)","description":"Monoclonal Antibody for studying KLRB1C mouse. Highly specific and rigorously validated in-house, NK1.1\/CD161 (E6Y9G) Rabbit Monoclonal Antibody (SignalFlex\u003csup\u003e™\u003c\/sup\u003e Pacific Blue\u003csup\u003e™\u003c\/sup\u003e Conjugate) (CST #77584) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nSignalFlex™ conjugates are produced using highly validated Cell Signaling Technology ® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays. Optimal dilutions\/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate.\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg\/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nNK1.1\/CD161 (E6Y9G) Rabbit mAb (SignalFlex™ Pacific Blue™ Conjugate) recognizes endogenous levels of total CD161c protein from C57BL\/6, SJL, and NZW mice, but not from BALB\/c and 129S6 mice. Mice from other strains have not been tested. This antibody is predicted to cross-react with CD161b and may cross-react with CD161a protein in some mouse strains.\nSpecies Reactivity: Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse NK1.1\/CD161 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCD161\/KLRB1 (Killer cell lectin-like receptor subfamily B member 1, also known as CLEC5B and NKR-P1A) is a type II transmembrane protein that is expressed on the majority of Natural Killer (NK) cells, NK T cells, and some T lymphocytes (1). CD161\/KLRB1 is also expressed on Th17 cells, promotes their generation, and modulates their function (2). Engagement with its ligand lectin-like transcript 1 (LLT1) inhibits NK cell function, while LLT1 and CD161\/KLRB1 interaction in the presence of a TCR signal enhances IFN-gamma production by T cells (3,4). There are several different CD161 isoforms in rodents and some function as activating receptors as well (5,6). There is a family of Klrb1 genes in rodents (7). PK136 antibody recognizes a specific epitope on mouse KLRB1b\/c, also referred to as NK1.1, and is commonly used for detection of mouse NK cells in certain mouse strains (CE, B6, NZB, C58, Ma\/My, ST, SJL, FVB). However, the epitope is absent in other mouse strains (BALB\/c, AKR, CBA, C3H, DBA, 129) (8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAI462337; CD161; CD161 antigen-like family member C; CD161c; killer cell lectin-like receptor subfamily A member 1C; killer cell lectin-like receptor subfamily B member 1C; Killer cell lectin-like receptor subfamily B member 1C; Klrb1b; Klrb1c; KLRBC; Ly-5; Ly-55c; Ly-59; Ly5; Ly55; Ly55c; Ly59; lymphocyte antigen 55 complex, locus C; Lymphocyte antigen 55c; lymphocyte antigen 59; natural killer cell receptor protein NKR-P1C; natural killer cell receptor-P1; Natural killer cell surface protein P1-40; natural killer cell-associated antigen 1; NK-; Nk-1; NK-1.; Nk-1.2; NK-RP1; Nk1; Nk1.; NK1.1; Nk1.2; NKR; NKR-; NKR-P1 40; NKR-P1.9; NKR-P1B; NKR-P1C; Nkrp; NKRP1; NKRP140; Nkrp1b; Nkrp1c\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M\nSENSITIVITY: Endogenous\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800529096873,"sku":"77584W2","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-77584w2","provider":"Iright","version":"1.0","type":"link"}