{"product_id":"cst-77748w2","title":"CST,  77748W2, Iba1\/AIF-1 (E4O4W) Rabbit Monoclonal Antibody (SignalFlex™ Alexa Fluor® 532 Conjugate)","description":"Monoclonal Antibody for studying AIF1\/Iba1. Highly specific and rigorously validated in-house, Iba1\/AIF-1 (E4O4W) Rabbit Monoclonal Antibody (SignalFlex\u003csup\u003e™\u003c\/sup\u003e Alexa Fluor\u003csup\u003e®\u003c\/sup\u003e 532 Conjugate) (CST #77748) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nSignalFlex™ conjugates are produced using highly validated Cell Signaling Technology ® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays. Optimal dilutions\/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate.\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg\/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nIba1\/AIF-1 (E4O4W) XP\nSpecies Reactivity: Human, Mouse, Rat, Hamster, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala139 of human Iba1\/AIF-1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nIonized calcium-binding adaptor molecule 1 (Iba1), also known as allograft inflammatory factor 1 (AIF-1), is an evolutionarily conserved cytoplasmic calcium binding protein containing a central pair of EF-hand calcium binding motifs (1,2). Iba1\/AIF-1 was originally cloned from activated macrophages in human atherosclerotic allogenic heart grafts undergoing chronic transplant rejection as well as from rat monocytes (3,4). Its function is not very well understood, but Iba1\/AIF-1 expression is upregulated in response to interferon-gamma and, therefore, could modulate macrophage-dependent immune response (3). As an F-actin-binding protein, Iba1\/AIF-1 may function to remodel the actin cytoskeleton and contribute to morphological changes that correlate with various microglial\/macrophage states (5). Iba1\/AIF-1 is also uniquely expressed in cells of monocytic lineage and is, therefore, widely used as a marker for microglia\/macrophages in the brain and other tissue (6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAIF-1; AIF1; Allograft inflammatory factor 1; G1; IBA 1; IBA-1; IBA1; interferon gamma responsive transcript; Ionized calcium-binding adapter molecule 1; IRT-1; IRT1; Protein G1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Hm Mk\nSENSITIVITY: Endogenous\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800529555625,"sku":"77748W2","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-77748w2","provider":"Iright","version":"1.0","type":"link"}