{"product_id":"cst-77814t","title":"CST,  77814T, PRPF6 (F9A2R) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying PRPF6. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, PRPF6 (F9A2R) Rabbit Monoclonal Antibody (CST #77814) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:200\nImmunofluorescence (Immunocytochemistry): 1:1600\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPRPF6 (F9A2R) Rabbit Monoclonal Antibody recognizes endogenous levels of total PRPF6 protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro202 of human PRPF6 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nPRPF6 is a critical spliceosomal protein that forms a bridge between the U5 and U4\/U6 small nuclear ribonucleoproteins (snRNPs) (1,2). It is phosphorylated by PRP4 kinase along with another PRPF protein of similar function, PRPF31 (3,4). The phosphorylation of these two proteins is a critical step in the stable U4\/U5\/U6 tri-snRNP complex (5). mutations have been described in retinitis pigmentosa, an autosomal-dominant disease that leads to retinal degeneration (6). PRPF6 has been implicated in cancer through its control of alternative splicing of key oncogenic isoforms, such as the ZAK kinase (7). PRPF6 has also been shown to interact with the androgen receptor (AR) and the AR splice variant 7 (AR-V7), thereby increasing AR transcriptional activity (8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nandrogen receptor N-terminal domain transactivating protein-1; Androgen receptor N-terminal domain-transactivating protein 1; ANT-1; ANT1; C20orf14; hPrp6; p102 U5 small nuclear ribonucleoprotein particle-binding protein; pre-mRNA processing factor 6; Pre-mRNA-processing factor 6; Prp6; PRP6 homolog; PRP6 pre-mRNA processing factor 6 homolog; PRP6 pre-mRNA processing factor 6 homolog (S. cerevisiae); PRPF6; putative mitochondrial outer membrane protein import receptor; RP60; SNRNP102; TOM; U5 snRNP-associated 102 kDa protein; U5-102 kDa protein; U5-102K\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 106\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799903129769,"sku":"77814T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-77814t","provider":"Iright","version":"1.0","type":"link"}