{"product_id":"cst-77860t","title":"CST,  77860T, SASH3\/SLY (F5S3X) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying SASH3. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, SASH3\/SLY (F5S3X) Rabbit Monoclonal Antibody (CST #77860) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nSASH3\/SLY (F5S3X) Rabbit Monoclonal Antibody recognizes endogenous levels of total SASH3\/SLY protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro53 of human SASH3\/SLY protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nEncoded by an X-linked gene, sterile alpha motif (SAM) and Src homology-3 (SH3) domain-containing 3 (SASH3), also called SH3-containing lymphocyte protein (SLY), is a putative lymphoid-specific signaling adaptor (1). One of the first clues that SASH3 plays a role in regulating the biology of lymphoid cells arose from research studies demonstrating that SASH3 is modified by phosphorylation subsequent to T cell receptor (TCR) engagement (2). Furthermore, the presence of SAM and SH3 domains strongly suggests that SASH3\/SLY participates in protein-protein interactions to regulate immune cell effector functions downstream of antigen receptor engagement. Indeed, naturally occurring loss-of-function mutations have been identified in human SASH3\/SLY that blunt activation of multiple key signaling nodes following TCR engagement, leading to an impairment of T cell proliferation and survival (3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n753P9; CXorf9; HACS2; SAM and SH3 domain containing 3; SAM and SH3 domain-containing protein 3; SASH3; SH3 protein expressed in lymphocytes homolog; SH3D6C; SLY\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 60\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799903391913,"sku":"77860T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-77860t","provider":"Iright","version":"1.0","type":"link"}