{"product_id":"cst-78954sf","title":"CST,  78954SF, OCA-T1 (E4T1K) Rabbit Monoclonal Antibody (BSA and Azide Free)","description":"Monoclonal Antibody for studying OCA-T1\/POU2AF2\/C11orf53. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, OCA-T1 (E4T1K) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #78954) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nThis product is the carrier free version of product #49316. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT\u0026amp;RUN or CUT\u0026amp;Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions\/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.\n\u003cb\u003eFormulation\u003c\/b\u003e\nSupplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 49316\n\u003cb\u003eStorage\u003c\/b\u003e\nStore at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze\/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nOCA-T1 (E4T1K) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total OCA-T1 protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro150 of human OCA-T1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nOCA-T1 is encoded by the gene ( ), which was identified in a single-cell RNA sequencing study to be expressed selectively in tuft cells from mouse and human tissues (1). The expression of OCA-T1 was observed to occur in a pattern strikingly similar to that of POU2F3, a master regulator of tuft cell development whose expression also defines a tuft cell-like variant (SCLC-P) of small cell lung cancers (2). Although containing no recognizable structural domains, OCA-T1 showed minor sequence similarity with OCA-B, a protein encoded by the gene and reported to be an activator of selected Class II POU domain transcription factors (3). Functional studies revealed that OCA-T1 forms a protein complex with POU2F3; these proteins furthermore share genomic binding sites and promote the expression of genes directly associated with tuft cell development. Lastly, Crispr\/Cas9-mediated deletion of in mice revealed that OCA-T1 is functionally required for normal tuft cell development (1). Collectively, these findings suggest that OCA-T1 is a critical component of the POU2F3-containing transcriptional complex that regulates the expression of genes governing tuft cell development.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nC11orf53; CK053; MGC50104; OCA-T1; POU class 2 homeobox associating factor 2; POU class 2 homeobox-associating factor 2; POU domain class 2-associating factor 2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 30\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799907356841,"sku":"78954SF","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-78954sf","provider":"Iright","version":"1.0","type":"link"}