{"product_id":"cst-79109s","title":"CST,  79109S, PAR1 (E9J9L) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying PAR-1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, PAR1 (E9J9L) Rabbit Monoclonal Antibody (CST #79109) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPAR1 (E9J9L) Rabbit Monoclonal Antibody recognizes endogenous levels of total PAR1 protein. This antibody recognizes a background band at 24 kDa of unknown origin.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp50 of human PAR1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nPAR1 (F2R) belongs to the PAR (Protease-Activated Receptor) family of G protein-coupled receptors. These membrane receptors are activated through amino terminal cleavage of the receptor by serine proteases such as thrombin, trypsin, or matrix metalloproteinases (1,2). Protease-mediated cleavage exposes the ‘tethered-ligand' fragment of the receptor; this binds to a second extracellular loop of the receptor and triggers receptor activation. Activated PAR1 couples to distinct G-protein complexes or arrestins, further relaying signals to downstream players (e.g., phospholipase C, Rho-kinase) (3-4). PAR1 function is necessary for normal physiological processes such as hemostasis, platelet activation, and thrombosis, whereas abnormal activation of PAR1 has been associated with inflammatory disorders, cardiovascular diseases, and metastasis (4-6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCF2R; coagulation factor II (thrombin) receptor; Coagulation factor II receptor; coagulation factor II thrombin receptor; F2R; HTR; PAR-1; PAR1; protease-activated receptor 1; Proteinase-activated receptor 1; Thrombin receptor; TR\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 47, 70-110\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799908536489,"sku":"79109S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-79109s","provider":"Iright","version":"1.0","type":"link"}