{"product_id":"cst-79132s","title":"CST,  79132S, IRF-1 (D5E4) Rabbit Monoclonal Antibody (Biotinylated)","description":"Monoclonal Antibody for studying IRF-1. Validated for Western Blotting. Highly specific and rigorously validated in-house, IRF-1 (D5E4) Rabbit Monoclonal Antibody (Biotinylated) (CST #79132) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 140 mM NaCl, 3 mM KCI, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg\/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nIRF-1 (D5E4) Rabbit Monoclonal Antibody (Biotinylated) recognizes endogenous levels of total IRF-1 protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro261 of human IRF-1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nInterferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak\/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, IRF-9\/ISGF3γ, IRF-3, IRF-4 (Pip\/LSIRF\/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8\/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E) (2).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nInterferon regulatory factor 1; IRF-1; IRF1; MAR\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 45-48\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800532111529,"sku":"79132S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-79132s","provider":"Iright","version":"1.0","type":"link"}