{"product_id":"cst-79145s","title":"CST,  79145S, GOLGA4\/GOLGIN-245 Antibody","description":"Polyclonal Antibody for studying GOLGA4. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, GOLGA4\/GOLGIN-245 Antibody (CST #79145) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nGOLGA4\/GOLGIN-245 Antibody recognizes endogenous levels of total GOLGA4\/GOLGIN-245 protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn2160 of human GOLGA4\/GOLGIN-245 protein. Antibodies are purified by peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe Golgi-associated protein golgin A1 (GOLGA1, golgin-97) was first isolated as a Golgi complex autoantigen associated with the autoimmune disorder Sjogren's syndrome (1). The golgin-97 protein contains a carboxy-terminal GRIP domain and is a commonly used trans-Golgi network (TGN) marker. All four known mammalian GRIP domain-containing proteins (golgin-97, golgin-245, GCC88, and GCC185) are found in the TGN, share extensive alpha-helical structure, and form homodimers (2). While all four golgin proteins localize to the TGN, they exhibit different membrane-binding abilities and are found in distinct TGN regions (3). Golgin-97 and golgin-245 are targeted to the TGN through an interaction between their GRIP domains and the Arl1 protein switch II region (4). Overexpression studies and siRNA assays with GRIP domain-containing proteins suggest that these proteins help to maintain TGN integrity and function by controlling localization of TGN resident proteins (5). By using a Shiga toxin B fragment (STxB)-based transport assay and an E-cadherin transport model system, golgin-97 and its effector Arl1-GTP were shown to play a role in trans-Golgi endosomal trafficking (6,7). Research studies also suggest that golgin-97 may play a role in poxvirus morphogenesis and maturation (8,9).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n256 kDa golgin; 72.1 protein; centrosome-related protein F46; CRPF46; GCP2; GOGA4; GOLG; GOLGA4; golgi autoantigen, golgin subfamily a, 4; golgin A4; Golgin subfamily A member 4; golgin-240; Golgin-245; MU-RMS-40.18; p230; Protein 72.1; Trans-Golgi p230\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 245\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799908241577,"sku":"79145S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-79145s","provider":"Iright","version":"1.0","type":"link"}