{"product_id":"cst-79404s","title":"CST,  79404S, Arginase-1 (D4E3M™) Rabbit Monoclonal Antibody (HRP Conjugate)","description":"Monoclonal Antibody for studying Arginase1. Validated for Western Blotting. Highly specific and rigorously validated in-house, Arginase-1 (D4E3M\u003csup\u003e™\u003c\/sup\u003e) Rabbit Monoclonal Antibody (HRP Conjugate) (CST #79404) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 136 mM NaCl, 2.6 mM KCl, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg\/ml BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibodies.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nArginase-1 (D4E3M™) Rabbit Monoclonal Antibody (HRP Conjugate) recognizes endogenous levels of total arginase-1 protein. This antibody conjugate does not cross-react with arginase-2.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val47 of human arginase-1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nL-arginine plays a critical role in regulating the immune system (1-3). In inflammation, cancer, and certain other pathological conditions, myeloid cell differentiation is inhibited leading to a heterogeneous population of immature myeloid cells, known as myeloid-derived suppressor cells (MDSCs). MDSCs are recruited to sites of cancer-associated inflammation and express high levels of arginase-1 (4). Arginase-1 catalyzes the final step of the urea cycle converting L-arginine to L-ornithine and urea (5). Thus, MDSCs increase the catabolism of L-arginine resulting in L-arginine depletion in the inflammatory microenvironment of cancer (4,6). The reduced availability of L-arginine suppresses T cell proliferation and function and thus contributes to tumor progression (4,6). Arginase-1 is of great interest to researchers looking for a therapeutic target to inhibit the function of MDSCs in the context of cancer immunotherapy (7). In addition, research studies have demonstrated that arginase-1 distinguishes primary hepatocellular carcinoma (HCC) from metastatic tumors in the liver, indicating its value as a potential biomarker in the diagnosis of HCC (8,9).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nARG1; ARGI1; arginase 1; Arginase-1; arginase, liver; Liver-type arginase; Type I arginase\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 40\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800532373673,"sku":"79404S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-79404s","provider":"Iright","version":"1.0","type":"link"}