{"product_id":"cst-79462t","title":"CST,  79462T, U2AF2 (E6M3G) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying U2AF2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, U2AF2 (E6M3G) Rabbit Monoclonal Antibody (CST #79462) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nU2AF2 (E6M3G) Rabbit Monoclonal Antibody recognizes endogenous levels of total U2AF2 protein. This antibody recognizes U2AF2 protein cleavage products around 43 and 46 kDa.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val275 of human U2AF2 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nU2 small nuclear RNA auxiliary factor 2 (U2AF2) is the large (65 kDa) subunit of the U2 auxiliary factor (U2AF) that plays an essential role in the splicing of pre-mRNA to generate functional mRNA transcripts. U2AF2 forms a heterodimer with the small (35 kDa) U2AF1 subunit to create the U2AF that recognizes the 3' splice site and facilitates spliceosome assembly (1-3). Research studies indicate that U2AF1 binds to the 3' splice site consensus AG dinucleotide at the intron-exon boundary while U2AF2 recognizes and binds the polypyrimidine tract upstream of the 3' splice site. These two steps ensure accurate spliceosome assembly at splice sites (4-6). Mutations in , which alter its splicing capabilities, have been described in various cancers (7,8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nhU2AF(65); hU2AF65; splicing factor U2AF 65 kD subunit; Splicing factor U2AF 65 kDa subunit; U2 (RNU2) small nuclear RNA auxiliary factor 2; U2 auxiliary factor 65 kDa subunit; U2 small nuclear ribonucleoprotein auxiliary factor (65kD); U2 small nuclear RNA auxiliary factor 2; U2 snRNP auxiliary factor large subunit; U2AF2; U2AF65\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 43, 46, 65\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799909650601,"sku":"79462T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-79462t","provider":"Iright","version":"1.0","type":"link"}