{"product_id":"cst-79561s","title":"CST,  79561S, CUT\u0026Tag pAG-Tn5 (Loaded)","description":"Enzyme for studying in the research area.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nPlease refer to CUT\u0026amp;Tag Assay Kit # 77552 for detailed use of this enzyme in the CUT\u0026amp;Tag assay. After cell permeabilization and primary and secondary antibody binding, resuspend cells in 50 μL of High Salt Digitonin Buffer containing 2 μL of pAG-Tn5 Enzyme (1:25 dilution). Incubate cell samples at room temperature for 1 hour, wash cells with High Salt Digitonin Buffer, and then perform the chromatin tagmentation.\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 22 mM HEPES pH 7.4, 44 mM NaCl, 44 μM EDTA, 0.4 mM DTT, 0.04% Triton X-100, and 50% glycerol. Store at -20°C and do not aliquot. This product is stable for 6 months.\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nSpecies Reactivity: All Species Expected\n\u003cb\u003eBackground\u003c\/b\u003e\nSimilar to Cleavage Under Targets and Release Using Nuclease (CUT\u0026amp;RUN), Cleavage Under Targets and Tagmentation (CUT\u0026amp;Tag) is a powerful technique used for probing protein-DNA interactions within the natural chromatin context of the cell (1-3). CUT\u0026amp;Tag has many of the same advantages as the CUT\u0026amp;RUN assay in that it provides a rapid, robust, and true low cell number protocol for detection of protein-DNA interactions in the cell. In addition, the CUT\u0026amp;Tag assay adds an adaptor DNA ligation step carried out by the pAG-Tn5 enzyme, in which an adaptor DNA is ligated directly to antibody-targeted chromatin DNA fragments in the cell. As a result, subsequent DNA library preparation is much faster and easier than library preparation following the CUT\u0026amp;RUN assay, free from DNA end repair, A-tailing, and adaptor ligation . CUT\u0026amp;Tag works very well for analyzing histone modifications, in addition to mapping some transcription factor and cofactor binding.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nC\u0026amp;T; CnT; CUT \u0026amp; Tag; cut and tag; CUT and Tag; CUT\u0026amp;Tag; cutandtag; histone modification","brand":"CST","offers":[{"title":"Default Title","offer_id":46800806052009,"sku":"79561S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-79561s","provider":"Iright","version":"1.0","type":"link"}