{"product_id":"cst-79799t","title":"CST,  79799T, SETD6 (F5G6P) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying SETD6. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, SETD6 (F5G6P) Rabbit Monoclonal Antibody (CST #79799) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:50 - 1:250\nImmunoprecipitation: 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nSETD6 (F5G6P) Rabbit Monoclonal Antibody recognizes endogenous levels of total SETD6 protein. This antibody detects an 85 kDa protein of unknown identity in some cell lines.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human SETD6 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nLysine methylation is a dynamic posttranslational modification that regulates protein function and various biological processes. SET domain-containing protein 6 (SETD6) is a mono-methyltransferase that promotes the methylation of multiple proteins involved in the regulation of gene expression programs (1). Unlike other lysine methyltransferases (KMTs), SETD6 was first discovered as a non-histone KMT and has been shown to methylate key regulatory factors such as RELA, TWIST1, and BRD4 (2-4). SETD6 can exist in monomer, oligomer, and higher-molecular-weight aggregate forms and its auto-methylation activity is key in stabilizing oligomerization and enzymatic activity (5). Moreover, SETD6 expression is misregulated in a number of cancers. Namely, SETD6 protein expression is upregulated in lung adenocarcinoma tumors, several bladder cancer cell lines, oral squamous cell carcinoma, and in samples from glioma patients (3,6-8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nFLJ21148; N-lysine methyltransferase SETD6; SET domain containing 6; SET domain containing 6, protein lysine methyltransferase; SET domain-containing protein 6; SETD6\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 55\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799911583913,"sku":"79799T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-79799t","provider":"Iright","version":"1.0","type":"link"}