{"product_id":"cst-8033t","title":"CST,  8033T, USP1 (D37B4) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying USP1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, USP1 (D37B4) Rabbit Monoclonal Antibody (CST #8033) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nUSP1 (D37B4) Rabbit Monoclonal Antibody recognizes endogenous levels of total USP1 protein.\nSpecies Reactivity: Human, Mouse, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu768 of human USP1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nUbiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzymes (DUB) action (1,2). There are five DUB subfamilies including the USP, UCH, OTU, MJD, and JAMM enzymes. USP1 belongs to the USP subfamily and is regulated in a cell cycle dependent manner by both transcriptional and ubiquitin-proteosomal mechanisms (3). USP1 is a nuclear protein and localizes to chromatin where it is specifically associated with FANCD2. USP1 deubiquitinates monoubiquitinated FANCD2, which plays an important role in DNA damage repair and Chk1 protein stability (3,4). Another important target of USP1 is PCNA. USP1 deubiquitinates monoubiquitinated PCNA, thereby negatively regulating PCNA-mediated translesion synthesis (TLS) during DNA repair (5). USP1 interaction with UAF1, a WD40 repeat-containing protein, leads to formation of an activated USP1\/UAF1 complex, which is required for the deubiquitinase activity of USP1 (6,7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nDeubiquitinating enzyme 1; hUBP; ubiquitin carboxyl terminal hydrolase 1; Ubiquitin carboxyl-terminal hydrolase 1; ubiquitin specific peptidase 1; ubiquitin specific processing protease 1; ubiquitin specific protease 1; Ubiquitin thioesterase 1; ubiquitin thiolesterase 1; Ubiquitin-specific-processing protease 1; UBP; UBP1; USP1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M Mk\nSENSITIVITY: Endogenous\nMW (kDa): 110\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799914959017,"sku":"8033T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-8033t","provider":"Iright","version":"1.0","type":"link"}